Microfluidic release of the rare cells captured by a filter with tapered holes

Rare cells such as circulating tumor and prenatal fetal can be captured by size-dependent filtration using a microfluidic device. Herein, we report method to release the with same device which consisted of microfabricated filter tapered holes. When cell containing flow passed through filter, could blocked filter. Afterward, reverse was applied cells. To increase efficiency capture release, holes used micro-particles sizes between smaller large hole diameters were added into flow. We found that when majority blocked, trans-filter resistance drastically increased most released. As proof concept, ovarian cancer SKOV3 used, showing high remarkable viability collected • Development rare Calculation pressure function percentage Demonstration at efficiency. after release.